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DSU DNA CORE CENTER
KingFisher Duo Prime
Isolates DNA and RNA from a variety of starting materials including cell-free body fluids, blood, bacteria, cell cultures, tissue and plant samples; with volumes ranging up to 5mL.
Principle of operation
The operating principle employed is inverse magnetic particle processing (MPP) technology. Rather than moving the liquids, the magnetic particles are moved row wise on the plate(s) or strip(s) containing specific reagents. Magnetic particles are transferred using magnetic rods covered with a disposable, specially designed plastic tip comb.
During the collection of the magnetic particles, the magnetic rod is fully inside the tip. The magnetic rods together with the tip comb move slowly up and down in the plate and the magnetic particles are collected onto the edge of the tips.
After collection of the magnetic particles, the magnetic rods together with the tip comb are lifted from the wells and transferred into the next wells containing the reagent, the magnetic rods together with the tip comb are lowered into the wells and the magnetic rods are lifted off. Magnetic particles are released by moving the tip comb up and down several times at considerably high speed until all the particles have been mixed with the substance in the next reaction.
Washing the magnetic particles is a frequent and an important processing phase. Washing is a combination of the release and collection processes in wells filled with washing solution. To maximize washing efficiency, the magnetic rods together with the tip comb are designed to have minimized liquid-carrying properties. To keep the magnetic particle suspension evenly mixed in incubating long-running reactions, the tip comb can be moved up and down in the solution.
The elution strip is typically used to perform the elution step that concentrates the purified sample. It is easy to pipette small volumes into the elution strip due to its low design.
MagMAX kits
MagMAX™ Pathogen RNA/DNA Kit
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Purification of RNA and DNA from viruses and easy-to-lyse bacteria and parasites, using magnetic particle technology in a 96-well format. PCR inhibitors are effectively removed in the process, making the resulting nucleic acid ideal for real-time PCR and RT-PCR applications.
MagMAX™ mirVana™ Total RNA Isolation Kit
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The kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.
MagMAX™ DNA Multi-Sample Ultra Kit
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The kit is optimized to isolate genomic DNA (gDNA) from a variety of samples, such as whole blood, buccal cells, saliva, urine, blood cards, mouth rinse, and tissue. gDNA purified with the kit is ideal for qPCR applications. The magnetic bead–based purification format allows you to easily scale from processing 12 to 500 samples a day, making it the ideal choice for pharmacogenomics (PGx) studies.
Bio-analyzer
During the preparation of samples for specific downstream applications such as next generation sequencing, gene expression and proteomics, it is important to determine the quality and quantity of your experimental starting material. The Agilent 2100 Bio-analyzer system has been acknowledged in over 35,000 scientific papers as an excellent tool for quality control of RNA and DNA samples. The Bio-analyzer can be used for DNA analysis in the following ways: sample QC in next generation sequencing (size distribution analyses and QC of PCR-amplified libraries), PCR validation and impurity check, restriction digestion analysis and food analysis.
DNA kits
DNA 1000 Kit
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For the separation, sizing and quantification of dsDNA fragments from 25 to 1000 bp
DNA 7500 Kit
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For the separation, sizing and quantification of dsDNA fragments from 100 to 7500 bp
DNA 12000 Kit
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For the separation, sizing and quantification of dsDNA fragments from 100 to 12000 bp
High Sensitivity DNA Kit
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For the separation, sizing and quantification of dsDNA samples of limited abundance and sized from 50 - 7000 bp
RNA kits
RNA 6000 Nano Kit
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For the analysis and quantitation of total and mRNA samples of 25 to 500 ng/µL in concentration.
Small RNA Kit
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For the analysis and quantitation of small RNA samples from 6 to 150 nt in size and 50 to 2000 pg/µL in concentration
High Sensitivity RNA Kit
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For the analysis of RNA samples of limited abundance down to 50 pg/µl of total RNA or 250 pg/µl of mRNA.
BluePippin
DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field (100bp – 50kb) for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user.
Principle of operation
Each sample lane is physically separate to eliminate the possibility of sample cross-contamination and features a branched configuration with three electrodes. DNA is separated along a gel column until the programmed fragment range reaches the branch point. The instrument then switches the active electrode to divert DNA into a membrane-bound buffer chamber. When the size range has been collected, the active electrode is switched back to the separation channel. The desired sample can be removed with a standard pipette.
Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.
Standard DNA Size Selection Cassettes
3% agarose, 100-250bp
2% agarose, 100-600bp
1.5% agarose, 250bp – 1.5kb
0.75% agarose, 1-50kb
High Pass Plus Cassettes, >15KB High Pass
View the BluePippin cassette reference chart to explore the DNA size selection options and run times. https://sagescience.com/wp-content/uploads/2018/09/BluePippin-Reference-Chart-460021-RevB-6_40_CD31-9_18_18.pdf
Qubit Fluorometric Quantification
With a Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample.
Principle of operation
Qubit Fluorometers detects fluorescent dyes that are specific to the target of interest. These fluorescent dyes emit only when bound to the target molecules, even at low concentrations. Both the Qubit 4 Fluorometer and the Qubit Flex Fluorometer are orders of magnitude more sensitive than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA (Figure 2). With a Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.
Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance.
More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA.
Uses as little as 1 μL of sample, even with very dilute samples.
Flexible options for exporting results: Wi-Fi, USB drive, or direct connection with a USB cable.
DNA kits
dsDNA high-sensitivity (HS)
Quantifies from 0.1 to 120 ng
dsDNA broad-range (BR)
Quantifies from 4 to 2,000 ng
single-stranded DNA (ssDNA)
Quantifies from 1 to 200 ng
RNA kits
RNA high-sensitivity
5 to 100 ng
RNA broad range
20 to 1000 ng
RNA extended range
200 to 10,000 ng
microRNA
1 to 1,000 ng
Covaris M220 Ultrasonicator
The M220 is a compact, easy-to-use system ideal for DNA shearing that eliminates operator-induced variations, improves recoveries, increases efficiency, and provides standardized results.
Tubes containing the sample are placed in a water bath that is temperature controlled. Focused acoustics put the minimum amount of energy into the DNA sample enabling isothermal processing and unbiased shearing. DNA is sheared into random fragments with the distribution centers selectable from 150bp to 5kbp.
For the fragment shearing guide https://covaris.com/wp-content/uploads/pn_010166.pdf
